Coding

Part:BBa_K4428004

Designed by: Dhwani Teckchandani   Group: iGEM22_IIT-Delhi   (2022-10-13)


Surface Display of PbrR-MBD using BclB

The lead binding domain of the protein PbrR can be expressed on the surface of bacteria with the help of the N-terminal domain of an anchor protein BclB for cell-surface adsorption of lead.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 321
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 321
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 321
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 321
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 112


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Categories
Parameters
None